In many bioscience applications, fluorescence-labeled proteins are used to analyze
cellular and biological functions. Here, fluorescein (FITC) and bovine serum albumin
(BSA) were used as model molecules. We tested the integration of a fluorescence
detector (FLD) into a semi-preparative FPLC system and evaluated the analysis of FITC
labeled BSA in comparison with classical UV/VIS detection. The use of a fluorescence
detector increases the sensitivity of the chromatographic analysis significantly.
Overlay of FITC-BSA peak measured with fluorescence (red) and UV 280 nm (blue). Sample 1 with 0.8 μg
Overlay of FITC-BSA peak measured with fluorescence (red) and UV 280 nm (blue). Sample 2 with 1.3 μg
|Substanzen||Fluorescein isothiocyanate isomer I (FITC), Bovine Serum Albumin (BSA)|
|Schlüsselwörter||SEC, FPLC, AZURA FPLC, fluorescence, BSA, FITC, fluorescein-labeled protein|
|CAS Nummer||3326-32-7, 9048-46-8|
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