KNAUER Blog – Fundamentals of High‑Performance LC

9 Articles

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Oligos Made Easy _ Part 6 Column Navigator

How to Navigate Through the Jungle of Columns? Struggling to Find the Right Column for Your Oligos? Oligonucleotides are complex, long-chain molecules with a very high charge density and a wide variet...

AEX HPLC Basics IP-RP KNAUER Oligonucleotide Purification Oligos SEC SepapureOligo Size Exclusion

Sep 29, 2025

Oligos Made Easy _ Part 5 SEC

Size Matters: Mastering Size Exclusion for Oligos 💡Why Use SEC in the Oligo Revolution? Oligonucleotides are taking center stage in everything from cutting-edge therapeutics to diagnostics and synthet...

HPLC Basics KNAUER Oligonucleotide Purification Oligos SEC Size Exclusion

Sep 15, 2025

Oligos Made Easy _ Part 4 HILIC

Stick With Me:The Cool Chemistry of HILIC for Oligonucleotide Analysis 💡Why Use HILIC in the Oligo Revolution? Oligonucleotides are the workhorses behind gene therapies, diagnostics, and research break...

HILIC HPLC Basics Hydrophilic Interaction Liquid Chromatography KNAUER Oligonucleotide Analysis Oligos Separation

Sep 1, 2025

Oligos Made Easy _ Part 3 IP-RP

Pair With Me: The Sleek Science of Ion Pair (IP)-Reversed Phase (RP) Oligonucleotide Analysis 💡Why Purity Matters in the Oligo Revolution? In the high-stakes world of molecular biology, oligonucleotid...

HPLC Basics IP-RP Ion-Pair Reagent Ion-Pair Reversed Phase Mode KNAUER Oligonucleotide Analysis Oligos Separation

Aug 18, 2025

Oligos Made Easy _ Part 2 AEX

Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis Oligonucleotides — short strands of DNA or RNA — are essential in modern molecular biology, playing a vital role in diagnostics, ...

AEX Anion Exchange HPLC Basics IEX Ion Exchange KNAUER Oligonucleotide Analysis Oligos Purification Separation

Aug 4, 2025

Oligos Made Easy _ Part 1 Overview

The Hitchhiker´s Guide to Creating Oligonucleotides from Start to Finish Back in 2020 we came up with an innovative system for making lipid nanoparticles (LNP), and it's completely changed things here...

Innovation KNAUER Oligonucleotide Analysis Oligonucleotide Synthesis Oligos Purification

Jul 21, 2025

In Bruges ...

The land of chocolate, beer, fries,waffles ... and fantastic separation science!!! With KNAUER and the seniors of the Application lab; Yannick Krauke, Ulrike Krop, Juliane Kramer The 54th edition of t...

Conference HPLC I Love HPLC Innovation KNAUER Science Together

Jul 7, 2025

Crab Cakes, Columns & Chromatograms

KNAUER at #ASMS2025 in Baltimore From June 1-5, 2025 the 73rd ASMS Conference on Mass Spectrometry and Allied Topics took place in the “Charm City” of Baltimore, Maryland, USA, famous for its historic...

Conference HPLC Innovation KNAUER Science Together

Jun 23, 2025

The importance of temperature control in preparative liquid chromatography.

Eluent Preheating in Preparative HPLC: Necessity and Benefits In preparative high-performance liquid chromatography (HPLC), where large columns and high flow rates are common, managing temperature is ...

Chromatography EluentPreheating HPLC KNAUER LabTechnology TemperatureControl

Apr 30, 2025