KNAUER Blog – Fundamentals of High‑Performance LC – AEX

28 Articles

easy ×

Oligos Made Easy _ Part 6 Column Navigator

How to Navigate Through the Jungle of Columns? Struggling to Find the Right Column for Your Oligos? Oligonucleotides are complex, long-chain molecules with a very high charge density and a wide variet...

AEX HPLC Basics IP-RP KNAUER Oligonucleotide Purification Oligos SEC SepapureOligo Size Exclusion

Sep 29, 2025

Oligos Made Easy _ Part 5 SEC

Size Matters: Mastering Size Exclusion for Oligos 💡Why Use SEC in the Oligo Revolution? Oligonucleotides are taking center stage in everything from cutting-edge therapeutics to diagnostics and synthet...

HPLC Basics KNAUER Oligonucleotide Purification Oligos SEC Size Exclusion

Sep 15, 2025

Oligos Made Easy _ Part 4 HILIC

Stick With Me:The Cool Chemistry of HILIC for Oligonucleotide Analysis 💡Why Use HILIC in the Oligo Revolution? Oligonucleotides are the workhorses behind gene therapies, diagnostics, and research break...

HILIC HPLC Basics Hydrophilic Interaction Liquid Chromatography KNAUER Oligonucleotide Analysis Oligos Separation

Sep 1, 2025

Let's talk about flash chromatography

Flash Chromatography Columns: A Comprehensive Overview and Benefits of Santai SepaFlash Flash chromatography is a popular purification technique used to separate mixtures of compounds quickly and effi...

Chromatography Columns Flash Columns

Sep 1, 2025

Oligos Made Easy _ Part 3 IP-RP

Pair With Me: The Sleek Science of Ion Pair (IP)-Reversed Phase (RP) Oligonucleotide Analysis 💡Why Purity Matters in the Oligo Revolution? In the high-stakes world of molecular biology, oligonucleotid...

HPLC Basics IP-RP Ion-Pair Reagent Ion-Pair Reversed Phase Mode KNAUER Oligonucleotide Analysis Oligos Separation

Aug 18, 2025

Oligos Made Easy _ Part 2 AEX

Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis Oligonucleotides — short strands of DNA or RNA — are essential in modern molecular biology, playing a vital role in diagnostics, ...

AEX Anion Exchange HPLC Basics IEX Ion Exchange KNAUER Oligonucleotide Analysis Oligos Purification Separation

Aug 4, 2025

Oligos Made Easy _ Part 1 Overview

The Hitchhiker´s Guide to Creating Oligonucleotides from Start to Finish Back in 2020 we came up with an innovative system for making lipid nanoparticles (LNP), and it's completely changed things here...

Innovation KNAUER Oligonucleotide Analysis Oligonucleotide Synthesis Oligos Purification

Jul 21, 2025

Everything to know about osmolality

Measuring Osmolality in Science and Industry: Applications, Methods, and the KNAUER Osmometer K-7400S Osmolality – the concentration of dissolved particles in a solution – is a fundamental parameter i...

Freezing Point Osmometer KNAUER KNAUER K-7400S Laboratory Measurement Technology Osmolality Osmometry

Jun 3, 2025

The importance of temperature control in preparative liquid chromatography.

Eluent Preheating in Preparative HPLC: Necessity and Benefits In preparative high-performance liquid chromatography (HPLC), where large columns and high flow rates are common, managing temperature is ...

Chromatography EluentPreheating HPLC KNAUER LabTechnology TemperatureControl

Apr 30, 2025

This is not an Easter Egg …

Seasonal update on liquid handling There is the German saying, “Der April macht was er will!” which in the figurative sense means that everything is unpredictable. Usually this is related to the weath...

Autosampler Liquid Handler LH 8.1 Robotic Cooler

Apr 17, 2025

Avoiding Common Syringe Filter Mistakes

A Practical Guide with Tips & Tricks to Master Syringe Filter Use Syringe filters are essential for chromatography and laboratory work by ensuring that samples are clean and free from particles. Howev...

Consumables HPLC HPLC Sample Prep Membrane Syringe Filter

Mar 31, 2025

Next HPLC – shop consciously

System specifications need an update In years past, buying an HPLC meant looking for the best system performance at the best price. You look at pump pulsation, injection reproducibility, detection op...

Feb 25, 2025

1
2
3