KNAUER Blog – Fundamentals of High‑Performance LC – AEX

9 Articles

Oligos × Oct 2025 ×

Oligos Made Easy _ Part 9 2D-LC

Twist of Two: 2D-LC for Oligos 💡Why Use 2D-LC for Oligo Analysis? In oligonucleotide analysis, precision isn't just important, it's critical. However, analyzing them is no easy task. Oligonucleotides ...

2D-LC Comprehensive Transfer HPLC Basics Heart-Cut Transfer KNAUER Oligonucleotide Analysis Oligos

Dec 8, 2025

Oligos Made Easy _ Part 8 Sepapure oliGO

A new specialist foroligonucleotides with advantages Oligonucleotides are the new superstars in medical and biological research, especially when it comes to highly specialized or personalized drugs....

AEX HPLC Basics IP-RP KNAUER Oligonucleotide Purification Oligos SEC SepapureOligo Size Exclusion

Nov 24, 2025

Oligos Made Easy _ Part 7 Large Scale AEX

The Secret to Pure Oligos: Why Anion Exchange Chromatography Rules at Scale Oligonucleotide therapeutics are on the rise. More and more short DNA and RNA drugs are entering clinical development and ev...

AEX Anion Exchange HPLC HPLC Basics IEX Ion Exchange Oligonucleotide Oligos Purification Separation

Oct 13, 2025

Oligos Made Easy _ Part 6 Column Navigator

How to Navigate Through the Jungle of Columns? Struggling to Find the Right Column for Your Oligos? Oligonucleotides are complex, long-chain molecules with a very high charge density and a wide variet...

AEX HPLC Basics IP-RP KNAUER Oligonucleotide Purification Oligos SEC SepapureOligo Size Exclusion

Sep 29, 2025

Oligos Made Easy _ Part 5 SEC

Size Matters: Mastering Size Exclusion for Oligos 💡Why Use SEC in the Oligo Revolution? Oligonucleotides are taking center stage in everything from cutting-edge therapeutics to diagnostics and synthet...

HPLC Basics KNAUER Oligonucleotide Purification Oligos SEC Size Exclusion

Sep 15, 2025

Oligos Made Easy _ Part 4 HILIC

Stick With Me:The Cool Chemistry of HILIC for Oligonucleotide Analysis 💡Why Use HILIC in the Oligo Revolution? Oligonucleotides are the workhorses behind gene therapies, diagnostics, and research break...

HILIC HPLC Basics Hydrophilic Interaction Liquid Chromatography KNAUER Oligonucleotide Analysis Oligos Separation

Sep 1, 2025

Oligos Made Easy _ Part 3 IP-RP

Pair With Me: The Sleek Science of Ion Pair (IP)-Reversed Phase (RP) Oligonucleotide Analysis 💡Why Purity Matters in the Oligo Revolution? In the high-stakes world of molecular biology, oligonucleotid...

HPLC Basics IP-RP Ion-Pair Reagent Ion-Pair Reversed Phase Mode KNAUER Oligonucleotide Analysis Oligos Separation

Aug 18, 2025

Oligos Made Easy _ Part 2 AEX

Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis Oligonucleotides — short strands of DNA or RNA — are essential in modern molecular biology, playing a vital role in diagnostics, ...

AEX Anion Exchange HPLC Basics IEX Ion Exchange KNAUER Oligonucleotide Analysis Oligos Purification Separation

Aug 4, 2025

Oligos Made Easy _ Part 1 Overview

The Hitchhiker´s Guide to Creating Oligonucleotides from Start to Finish Back in 2020 we came up with an innovative system for making lipid nanoparticles (LNP), and it's completely changed things here...

Innovation KNAUER Oligonucleotide Analysis Oligonucleotide Synthesis Oligos Purification

Jul 21, 2025