HPLC | ULDC | Analytical 862 bar systems

KNAUER AZURA® HPLC 862 bar system key features

With the 862 bar system, you choose the most widespread system solution of the KNAUER AZURA family.
We recommend this system for all classical applications and column types, as well as for faster and optimized methods with shorter columns and run times.

The complete solution for your daily HPLC routine. Easily upgradable to an ultra low dispersion chromatography (ULDC) system
Covers most analytical applications due to optimal continuous working conditions in the range of 0.1–4.0 ml/min at up to 862 bar
Use classical HPLC columns just as well as fast analysis columns packed with 3 µm particles or even core shell materials
Typical applications include food, pharmaceutical and environmental analyses

Choose this system for reliable and robust analyses!

If you are looking for higher speed, high sample throughput or highest peak resolution, take a look at the KNAUER AZURA UHPLC systems.

UHPLC systems

Specifications

Back pressure range	862 bar max. at up to 2 ml/min, 400 bar at up to 10 ml/min
Flow rate range	0.001–10 ml/min
Recommended continuous flow rate	Up to 5 ml/min
Available pump systems	Isocratic/Binary HPG/Quaternary LPG
Biocompatibility	Optionally available for backpressure range up to 400 bar
Injection options	Autosampler, Liquid handler, manual, automatted via electrical injection valve
Injection volume range	0.1 µl–5000 µl depending on sample loop
Sample capacity	108 standard HPLC vials (1.5 ml) or up to 768 positions when equipped with microtiter plates, with liquid handler up to 390 HPLC vials
Column thermostatting range	5–85 °C
Eluent preheating	Optional cartridge for eluent pre-heating
Column switching options	Optional switching between up to 8 columns
Choice of detectors	DAD 190–700 nm, DAD 190–1000 nm, RID, UVD, MWD, FLD, ELSD, MS
Choice of software packages	ClarityChrom® CDS, OpenLAB® CDS EZChrom Edition, Mobile Control, Chromeleon™ 7.2

Eluent delivery

Binary high-pressure gradient (HPG) pump system
Quaternary low-pressure gradient (LPG) pump system
Isocratic pump system

Binary high-pressure gradient (HPG) pump system

The AZURA® P 6.1L binary pump contains two identical high pressure pumps, a 2×2-channel inlet solvent selection valve and the low-volume AZURA® mixer. The integrated degasser and AZURA® inline filter are completing this analytical AZURA® P 6.1L HPLC pump and turn it into a work horse in the lab. Active pulsation compensation significantly improves detection limits of trace components by reducing fluctuation of the baseline.

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AZURA Pump P 6.1L HPG
Quaternary low-pressure gradient (LPG) pump system

The AZURA® P 6.1L quaternary pump contains one high pressure pump and an integrated LPG mixing block with a 4-channel inlet solvent selection valve and a low-dwell volume AZURA® mixer. The integrated degasser and AZURA® inline filter are completing this analytical AZURA® P 6.1L HPLC pump and turn it into a work horse in the lab. The AZURA® mixer combines high mixing performance with a low dead volume. The user-changeable mixer is available in different volumes (50/100/200/400/600 μl) allowing best possible adaption to any application.

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AZURA Pump P 6.1L LPG
Isocratic pump system

The AZURA® P 6.1L isocratic pump is the easiest solution and can be applied, whenever an isocratic run is needed. It completes systems with refractive index detector. The isocratic pump comes in a very cost effective version or in a more sophisticated version with degasser and solvent selection valves for switching between different eluents. Chose between specialised pump heads for reversed phase and normal phase applications.

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Sample Injection

Autosampler
Liquid handler
Manual injection valve
Electrically driven injection valve

Autosampler

The autosampler AZURA AS 6.1L can inject from up to 768 positions when equipped with microtiter plates or from up to 108 standard 2 ml sample vials. It is equipped with an ILD^TM valve, consisting of a rotor-stator combination with a central port for depressurizing the sample loop before receiving the sample. This way, the sample is not diluted with solvent. A cooling/heating option (4–40 °C) is optionally available.

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Autosampler AZURA AS 6.1L
Liquid handler

The Liquid handler LH 8.1 offers automatic sample preparation to speed up your workflow. No sample loss and high precision injection of small and large sample volumes are provided. High-throughput with up to 4 robotic coolers with max. of 24 racks for up to 390 HPLC vials or well plates. Washing station with two different wash solvents minimizes carry-over. Flexibility of modules and functions according to your needs.

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Manual injection valve

KNAUER Manual injection valves V 4.1 are the easiest and most cost- effective way to inject your sample. Equippable with different sample loops, the manual injection valve can be used for a wide range of injection volumes.

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Electrically driven injection valve

Whenever you need higher reproducibility and want to get user-independent results, a manual injection valve can be upgraded to an electrically switching injection valve using the KNAUER smart valve drive VU 4.1. The sample can still be applied manually, but the injection into the HPLC system is automatted.

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Column selection and thermostat

Column thermostat
Column switching valves

Column thermostat

The forced air column thermostat AZURA CT 2.1 is capable of heating or cooling from 5 to 85 °C. The powerful fan and robust peltier element keep the column at a very stable temperature, thus allowing reproducible analysis results. Optional eluent preheating cartridges can be installed.

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AZURA Column Thermostat CT 2.1
Column switching valves

KNAUER valves V 4.1 and the KNAUER valve drive VU 4.1 are designed for a wide range of chromatographic applications. Flexibility is provided by choice of different materials and sizes as well as drivers for various software packages.

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Detection

Diode array detectors (DAD or PDA)
Mass Detector for MS/MS
Variable or multiple wavelength detectors (MWD or VWD)
UV/VIS detectors with single variable wavelength
Refractive index detection (RI)
Fluorescence detection (FLD)
Evaporative light scattering detection (ELSD)

The AZURA Diode array detectors (DAD) are high-end diode array detectors with a wavelength range from 190 to 1000 nm for the AZURA DAD 6.1L and 190 - 700 nm for the AZURA DAD 2.1L. The intelligent temperature management of both devices enables fast operational readiness after startup. It also minimizes baseline drift. Due to the frontally accessible deuterium and halogen lamp, the device is easy to service.

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Detectors: AZURA Detector DAD 2.1L
The SCIEX Triple Quad™ 4500 MS/MS system offers superior quantitative performance with high sensitivity combined with excellent robustness and long-term stability. Designed for food, environmental, forensic and clinical research, this MS/MS system enables test development for even the most demanding applications.

Workhorse triple quad mass spectrometer for dependable everyday use

High sensitivity for food and environmental contaminant screening and target quantitation

High scan speed for reliable quantitation of sharp UHPLC peaks

Simple and fast data processing powered by SCIEX OS software

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The AZURA MWD 2.1L is an 8 channel, sensitive multiwavelength detector combining high performance with easy handling at an affordable price. The newly developed optical unit and intelligent temperature management ensure for maximum sensitivity combined with minimal baseline drift.

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Detectors: AZURA Detector MWD 2.1L
The UV/VIS Detector AZURA UVD 2.1L is a competitively priced HPLC spectrophotometer for routine HPLC applications including fast LC methods. It comes with an installed deuterium lamp which covers a wavelength range from 190 to 750 nm. Due to a smart design the flow cell is easily accessible and can be changed very quickly.

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AZURA UVD 2.1L detector
The AZURA RID 2.1L is a sensitive and competitively priced differential refractometer. It is suitable for detecting compounds with little or no UV activity such as alcohols, sugars, lipids or polymers. The intelligently designed optical unit with advanced temperature control ensures high sensitivity, fast baseline stabilization, and excellent reproducibility. The wide linear dynamic range, its low internal volume of 43 µl from the inlet to the flow cell and 10 ml/min maximum flow rate make the AZURA RID 2.1L the perfect choice for most laboratory tasks.

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AZURA Detector RI 2.1L
The fluorescence detectors RF-20A and RF-20Axs provide world-class sensitivity, excellent maintainability and diverse validation / support functions. They support a wide range of applications in the wavelength range of 200 to 750 nm for the RF-20Axs and 200 to 650 nm for the RF-20A from conventional analysis to high-performance analysis.

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As an universal detector, an ELSD offers numerous possibilities for detecting substances that have few or no chromophores. Since the eluents are evaporated, the use of non-UV-compatible solvents poses no problems and the ELSD is gradient compatible. KNAUER offers different models for different application tasks.

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Software solutions

Mobile Control
OpenLAB® CDS EZChrom Edition
ClarityChrom® CDS
Chromeleon™ 7.2

Mobile Control

The Mobile Control is an intuitive and cost-effective software solution for controlling and monitoring your AZURA devices and systems. The clear system overview provides instant information on key parameters and system status. Adjust and operate single devices or even whole systems with a few fingertips. The well-structured block programming assists you to clearly and quickly automate device control. A smart user management makes the Mobile Control safe. Device parameters can only be changed by authorized operators.

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OpenLAB® CDS EZChrom Edition

Basic Workstation license, includes System Suitability, Fraction Collector Control and Software Maintenance Agreement

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ClarityChrom® CDS

ClarityChrom is an easy-to-use chromatography data system for workstations. The optional control extensions for GC, PDA and GPC allow to use the software for a wide range of applications.

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Chromeleon™ 7.2

Thermo Scientific™ Dionex™ Chromeleon™ is one of the most wide-spread chromatography data systems. Its intuitive handling benefits laboratory workflow and the highly developed algorithms simplify data processing. It offers a broad range of third-party drivers and can be easily used with existing HPLC systems. KNAUER offers drivers for a lot of its devices.

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