The flexible protein purification platform

Design your FPLC system according to your purification task!

Complete solutions for FPLC (Fast Protein Liquid Chromatography) on a small footprint: AZURA® FPLC systems combine flexibility and reliability. The biocompatible/metal-free AZURA® FPLC is the perfect choice for your protein purification task. 

Design your AZURA Bio purification system to your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection as well as fraction collection enable the user to automate the purification process.
A range of different detectors let you keep track of your target molecules. A wide flow rate range and compatibility with columns from all vendors offer maximum flexibility. The intuitive software PurityChrom® combines all the advantages of a versatile purification software.

What is Fast Protein Liquid Chromatography (FPLC)?

FPLC is a type of liquid chromatography for the purification of large biomolecules such as proteins or DNA. External factors such as high temperature, high pressure, extreme pH, solvents or certain metals can affect the protein structure and are therefore avoided in FPLC. The column materials used, such as agarose or polymer materials, are also quite sensitive to pressure fluctuations and air bubbles.
Our systems take these requirements into account and can be flexibly configured for your purification challenges!

You choose the method-
We have the Bio purification system for you

Configure your perfect FPLC system

Build a custom solution based on your requirements. Just pick the components that match your method parameters.
From simple to complex, from Lab to Pilot scale: Design your AZURA® FPLC system according to your purification task!
AZURA Bio Lab allows you to create FPLC systems with highest independence. Just pick your modules and build-up the system yourself. Continue flexibility with intuitive PurityChrom® software.

FPLC Valves for your Lab system at one glance

FPLC Software PurityChrom®

The PurityChrom® is a powerful software to control your FPLC system. Get familiar with PurityChrom® in shortest time and with no effort due to the intuitive and clearly structured user interface. Choose a time- or volume based workflow by just clicking one button. Create methods with highest flexibility to realize complex application without losing easy handling. Offline licenses for creating methods and data evaluation are for free.

Made in Germany - the new FPLC columns

Discover the new prepacked FPLC columns based on Agarose and Dextran. KNAUER offers prepacked FPLC columns and resins for all common FPLC applications: Size Exclusion Chromatography (SEC), Affinity Chromatography (AC) and Ion-Exchange Chromatography (IEX)

Bio purification accessories and FPLC spare parts

Sometimes it is the little things that produce the big results. In addition to systems and devices, KNAUER offers a wide range of Accessories and Supplies. These parts are compatible with all commonly used HPLC or FPLC systems, columns, pumps, as well as detectors and were designed to simplify everyday laboratory life.

Accessory Features Benefit
• Contains two pressure sensors
• Automatic determination of pressure difference with PurityChrom®
• Connect 1/16” or 1/8” tubings
• Up to 250 ml/min and 60 bar
Monitor pressure over the
column bed and protect
column from damage
Air Sensor • Detect end of buffer or end of sample with PurityChrom®
• Up to four air sensors per system
• For transparent tubings with 1/16” or 1/8” or 1/4” outer diameter
Protect column from air
damage and support
automation ( e.g. sample
• Attach air sensor, pressure control, AZURA Organizer or your
interface box to the side panel of your AZURA L device
Organize your system.
• Attach columns from 5 mm to 26 mm diameter, falcon tubes, a
back pressure regulator or a pH flow cell
Organize accessories directly
at the system and reduce
dead volume
Back pressure
regulator (BPR)
• Apply a constant back pressure to your system
• Freely adjustable between 1-20 bar or 20-103 bar
Prevent formation of air
bubbles after the column
which disturb detector signal

Scale-up from Lab to Pilot

Choose the Pilot series if you want to increase your productivity even more. Upscale our Lab configuration with same flexibility, software PurityChrom® but minimal footprint. Just transfer and upscale your methods. Flow rates up to 1000 ml/min and loads up to several grams are possible.

AZURA® Bio purification (FPLC)

The complete brochure gives you an excellent overview of protein purification with the flexible AZURA FPLC systems. Get to know the components of the FPLC Bio Systems and the different possibilities in detail.
We have designed our systems to meet your purification challenges!

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We are happy to answer your questions about our products and services.


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