YMC and KNAUER
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Applying AEX and IP-RP
Purification and quality control of oligonucleotides
Oligonucleotides represent a critical class of biopharmaceuticals, with their importance continuing to escalate. However, the purification process poses significant challenges due to the high degree of similarity among impurities generated during synthesis.
This seminar presents a comprehensive approach to the large-scale purification of an oligonucleotide utilizing anion exchange chromatography (AEX), complemented by rigorous quality control measures through ion-pairing reversed phase chromatography (IP-RP).
Speakers
Dr. Ulrike Krop, KNAUER Wissenschaftliche Geräte GmbH
Dr. Daniel Eßer, YMC Europe GmbH
Content
- Strategies for large-scale AEX purification: Designing an effective preparative system and key features
- Advantages and drawbacks of utilizing AEX for preparative purification
- Key elements in developing IP-RP methods are the stationary phase, ion-pairing reagent, and solvent conditions.
- Optimizing analytical performance by employing bioinert column materials
Case studies for a GLP-1 agonist and a peptide hormone
Method development and scale-up in peptide purification
Peptides have established themselves as pivotal entities within the biopharmaceutical landscape. The inherent variability in their chain lengths and amino acid sequences facilitates a broad spectrum of pharmaceutical applications, while simultaneously necessitating precise methodologies for their production and purification.
This seminar is intended to furnish participants with the advanced knowledge required for the development of sophisticated peptide purification methodologies, informed by contemporary case studies, including a GLP-1 agonist and a peptide hormone.
Speakers
Julia Bartmann, YMC Europe GmbH
Dr. Yannick Krauke, KNAUER Wissenschaftliche Geräte GmbH
Content
- Selecting the perfect stationary phase: key factors to evaluate and the necessity of thorough testing
- Factors to consider in method development: optimizing conditions through careful choice of solvents, pH levels, and buffer systems
- From broad analysis to precise gradient: enhancing a reliable preparative technique
- Scaling Up Linearly: Grasping the Concept, Designing the System, and Collecting Samples
