The demand of pure cannabidiol (CBD) and other cannabinoids is increasing and therefore methods are needed that allow simultaneous purification of several cannabinoids from the same sample i.e. cannabis extract, paste or oil. Preparative HPLC is a well-established method for purification of target substances from natural substrates in high purities. In this application, a preparative HPLC method was developed for purification of CBD, Δ9-THC and CBC from a commercial CBD rich oil. All three cannabinoids were purified with high purity thus increasing the purity of the main component CBD from 72% to 93%.
Overlay chromatograms of CBD oil sample (blue) and mixture of 16 Cannabinoids (red).
Volume overload study with methanol method on C18 10 μm particle.
Preparative purification of CBD.
CBD oil and seeds
photo: pixabay/CBD-Infos-com
| Method | HPLC |
|---|---|
| Mode | RP |
| Substances | Cannabinoids |
| Key words | Cannabis, hemp, purification, scale-up, preparative LC, CBD purification, CBDV, CBG, CBD, CBN, Δ9-THC, CBL, CBC |
| CAS number | 24274-48-4, 2808-33-5, 13956-29-1, 521-35-7, 1972-08-3, 21366-63-2, 20675-51-8 |
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