In order to enable commercial use in the quality assurance of the crude products and for the production of high-purity reference compounds, the plant extract must be purified, since matrix components stress the separation column and reduce the loadability.
In this application, we are investigating the matrix removal efficiency of preparative online SPE and the achievable yield of pure rebaudioside A and stevioside.
Steviol glycosides are the main sweetening compounds in Stevia rebaudiana. Due to their up to 400 times higher sweetening power compared to sucrose or glucose they are often used as natural sugar substitutes.
Overload experiments on preparative column, 200 μL (red), 500 μL (blue), 2000μL (green); 1) rebaudioside A, 2) stevioside, blue bars – matrix, 25°C, 22 ml/min
Preparative online SPE, 10 mL loading; 1) rebaudioside A, 2) stevioside, blue bars – matrix, 25°C, 22 mL/min
Fraction analysis of preparative online-SPE purification (Fig.2) of rebaudioside A (1) and stevioside (2); A) fractionation of target peak, 5 mL fractions B) F3 (blue), F4 (red), F5 (green), F6 (light blue); C) F7 (red dashed), F10 (blue dashed), F12 (gre
| Method | Preparative HPLC |
|---|---|
| Mode | RP |
| Substances | rebaudioside A, stevioside |
| Key words | Stevia, sweetener, steviol glycosides, online SPE, solid phase extraction, purification, preparative HPLC, sample preparation |
| CAS number | 58543-16-1, 57817-89-7 |
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