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Start up, shut down - Screening of pressure sensitive columns with the 8 port 2-position valve

Application No.: VTN0008

Start up, shut down - Screening of pressure sensitive columns with the 8 port 2-position valve

Using valves in FPLC is most often necessary due to manual injection, column switching or fractionation. But also common analytical HPLC applications can be improved by adding valves. Widespread analytical application areas are for example column screenings to find the suitable column. A large variety of valves is available, but not all are suitable for the screening of pressure sensitive columns. This note describes an application of a KNAUER 8 port 2-position valve in a screening process with Eurokat columns.

Technical Data

Application details

Method HPLC
Mode Ion exclusion, Ligand exchange
Substances Cellobiose, mannitol, glucose, xylose, xylitol, glycerin, arabinose
Key words Eurokat, carbohydrates, column screening, sugar, PSDVB, polymer column, degree of polymerization, RID, refractive index
CAS number 528-50-7, 87-78-5, 58367-01-4, 609-06-3, 87-99-0 , 56-81-5 , 147-81-9
Version 1/10-2020

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