EN DE

How can reversed phase HPLC columns be cleaned and regenerated?

This is our recommended regeneration procedure for RP packings C18, C8, C4, C1, C30, CN and Phenyl stationary phases:

Flush the column with 20 column volumes of water
Flush the column with 20 column volumes of acetonitrile
Flush the column with 5 column volumes of isopropanol
Flush the column with 20 column volumes of heptane
Flush the column with 5 column volumes of isopropanol
Flush the column with 20 column volumes of acetonitrile

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