The determination of carbohydrates can sometimes be challenging. Not all columns are able to separate monomers, dimers, and oligomers at the same time. The KNAUER Eurospher II Amino (NH2) phase applied in hydrophilic interaction liquid chromatography (HILIC) mode can solve this task. Even carbohydrates with a high degree of polymerization (DP) can be separated.
Abstracted illustration of a bonded amino group to silica.
Chromatogram of sugar and inulin-type FOS standard, 2.50 mM. 1 - injection peak, 2 - glucose, 3 - sucrose, 4 - 1-kestose, 5 - 1,1-kestotetraose, 6 - 1,1,1-kestopentaose
Chromatogram of FOS from chicory, 50 mM. 1 - fructose, 2 - DP2, 3 - DP3, 4 - DP4, 5 - DP5, 6 - DP6, 7 - DP7
FOS generated from bacterial inulosucrase after 0.5 hours. 1 - injection peak, 2 - sample matrix, 3 - glycerol, 4 - glucose, 5 - sucrose, 6 - DP3, 7 - DP4, 8 - DP5, 9 - DP6, 10 - DP7, 11 - DP8
FOS generated from bacterial inulosucrase after 1 hour. 1 - injection peak, 2 - sample matrix, 3 - glycerol, 4 - glucose, 5 - sucrose, 6 - DP3, 7 - DP4, 8 - DP5, 9 - DP6, 10 - DP7, 11 - DP8, 12 - DP9, * - unidentified substance
- injection peak, 2 - sample matrix, 3 - glycerol, 4 - fructose/glucose, 5 - sucrose, , 6 - DP3, 7 - DP4, 8 - DP5, 9 - DP6, 10 - DP7, 11 - DP8, 12 - DP9, 13 - DP10, 14 - DP11, 15 - DP12, 16 - DP13, 17 - DP14, 18 - DP15, 19 - DP16, 20 - DP17, * - unidentif
| Method | HPLC |
|---|---|
| Mode | HILIC |
| Substances | glucose, sucrose, 1-kestose, glycerol, 1,1-kestotetraose, 1,1,1-kestopentaose, carbohydrates from DP3 to DP17 |
| Key words | HILIC, NH2, carbohydrates, sugar, FOS, fructooligosaccharides, bacterial inulosucrase, inulin-type FOS |
| Version | 1/10-2020 |
Article No.: 25XE190E2J
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