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Which information do I get from the theoretical plate number?

The number of theoretical plates correlates with the particle size. The smaller the particle, the higher the number of theoretical plates can be. A typical number of plates for 5 µm particle size is about 80.000 N/m, for 3 µm 130.000 N/m. Columns with high plate numbers are considered to be more efficient than columns with lower plate numbers. A column with a high number of plates will have a narrower peak at a given retention time than a column with a lower number of plates.

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

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