Which are the most important parameters for HPLC column evaluation?

There are many readable parameters you can get out of your chromatogram, for example retention time, peak symmetry, resolution, capacity, peak width and height, peak area, theoretical plates and so on. The most important column parameters for evaluation are the retention time, peak symmetry and theoretical plates. Retention time to prove the reproducability of measurements. Peak symmetry to estimate how good the column bed is packed. A peak symmetry value of 1.0 is optimal. Theoretical plate numbers to determine the effectiveness of the HPLC column.

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