What kind of stationary phases are typically used in RP columns?

The stationary phases for RP columns are surface modified silica gels or polymers with bounded alkyl chains which have hydrophobic/covalent properties. The longer the linked chain, the more hydrophobic the phase will be. For the surface treatment commonly silanols are used. The most typical and best known reversed stationary phase is the C18 modification.
Available KNAUER modifications of Eurospher II materials are C4, C8, C8 A, C18, C18 A, C18 H, C18 P and Phenyl.


We separate molecules and unite people.

Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB

We are an established partner in science. Today and in the future.​​​​​​

Do you need more information?

Please contact us!

Follow us