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What are typical pH limits for HPLC columns?

Standard silica-based phases are stable within a pH range from 2-8. Columns with special modifications or endcapping can be operated in an enlarged pH range. Materials like Eurospher II C18 H and Eurospher II C18 P are suited for applications with higher pH values (pH 9).

Polymer columns, like KNAUER Eurokat, are extremely stable over the whole pH range.

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

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