In biorefineries, bacteria and yeast are used for the fermentation of C5 and C6 sugars into bio-ethanol. The by-products from bio-ethanol production are also of value and can be used in other applications. To determine the most suitable column for the analysis of fermentation samples, sugar and sugar alcohol standards were screened using ion exchange Eurokat columns. Measurements using these columns enable the identification and quantification of fermentation broth components, as well as the determination of any impurities that may be present. Screenings using Eurokat Ca, Na, Pb and H columns provided high detection and separation performance for sugars and sugar alcohols. This performance demonstrated that Eurokat columns are suitable for the separation of biorefinery products, as well as for other applications.
Measurement of sugar standards on the Eurokat Ca column. Flow rate 0.5 ml/min, temperature 75 °C, sample concentration 2 mg/ml, injection volume 20 µl
Measurement of sugar standards on the Eurokat Na column. Flow rate 0.5 ml/min, temperature 75 °C, sample concentration 2 mg/ml, injection volume 20 µl.
Measurement of sugar standards on the Eurokat Pb column. Flow rate 0.5 ml/min, temperature 75 °C, sample concentration 2 mg/ml, injection volume 20 µ
Measurement of sugar standards on the Eurokat H column. Flow rate 0.7 ml/min, temperature 60 °C, sample concentration 2 mg/ml, injection volume 20 µl
Glucose (red) and mannitol (blue) measured with Eurokat Na (above) and Eurokat Ca (below) columns. Flow rate 0.5 ml/min, temperature 75 °C, sample concentration 2 mg/ml, injection volume 20 µl.
Arabinose (red) and fructose (blue) measured with Eurokat Na (above) and Eurokat Ca (below) columns. Flow rate 0.5 ml/min, temperature 75 °C, sample concentration 2 mg/ml, injection volume 20 µl
Arabinose (red) and fructose (blue) measured with Eurokat Pb (above) and Eurokat Ca (below) columns. Flow rate 0.5 ml/min, temperature 75 °C, sample concentration 2 mg/ml, injection volume 20 µl.
Rhamnose (red) and glucose (blue) measured with Eurokat H (above) and Eurokat Ca (below) columns. Ca: flow rate 0.5 ml/min, temperature 75 °C; H: flow rate 0.7 ml/min, temperature 60 °C; sample concentration 2 mg/ml, injection volume 20 µl.
Instrument composition
| Method | HPLC |
|---|---|
| Mode | Ion exclusion, Ligand exchange |
| Substances | Sucrose, Fructose, Galactose, Maltose monohydrate, Mannose, Glucose, Xylose, Arabinose, Cellobiose, Rhamnose monohydrate, Lactose monohydrate, Mannitol, Xylitol, Arabitol, Glycerol, Sorbitol |
| Key words | Sugar screening, sugars, sugar alcohols, Eurokat columns, fermentation |
| CAS number | 57-50-1, 57-48-7, 59-23-4, 6363-53-7, 3458-28-4, 14431-43-7, 58-86-6, 5328-37-0, 528-50-7, 10030-85-0, 64044-51-5, 69-65-8, 87-99-0, 7643-75-6, 56-81-5, 50-70-4 |
| Version | Version 1/10/2021 |
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