When separating and purifying molecules by liquid chromatography (LC), it is necessary to develop the method with a preparative high-performance liquid chromatography (HPLC) system. The purification process is often verified for quality and quantity of the products by analytical HPLC. In this case, two LC systems, a preparative and an analytical, are needed for a purification process and the following analysis.
In this technical note a combination of these two systems is shown. It describes in detail the system set up and the different method steps. The system is capable of isolating a single substance from a mixture and afterwards performing an automated purity analysis. For proof of concept, caffeine was purified from paracetamol and analysed within the same system.
Peparative and analytical column
|Method||Preparative and analytical HPLC|
|Key words||Hybrid system, preparative hplc, analytical hplc, purification, purify and analyze, caffeine, paracetamol|
|CAS number||Paracetamol: CAS 103-90-2; Caffeine: CAS 58-08-2|
|Version||1 / 10-2020|
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