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Separation of AQC Derivatized Amino Acids with Fluorescence Detection

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Application No.: VFD0064J

Separation of AQC Derivatized Amino Acids with Fluorescence Detection

Eurospher II 100-3 C18, 150 x 3 mm ID

Order No. 15CE181E2G

Vertex Plus columns, 30x4 mm, 50x4 mm, 100x4 mm, 120x4 mm, 125x4 mm, 150x4 mmFor a link to the column see below: Related products

Technical Data

Application

Method HPLC

Separation conditions

Eluent A: 50 mM Na-Acetat pH 5.75 B: 50 mM Na-Acetat pH 6/Acetonitrile 30:70
Gradient 0 – 10.13 min 5 % – 10 % B 10.13 – 16.0 min 10 % B – 25 % B 16.0 – 21.9 min 25 – 32 % B
Flow rate 0.8 ml/min
Mode Matrix
Injection volume 1 µl (1.176 pmol/µl, Cys 0.588 pmol/µl)
Temperature 45 °C
Additional procedure Manual AQC-derivatization of amino acid standard.
Detection Fluorescence (Ex 250 nm, Em 395 nm)
Substances Alanine, Arginine, Aspartic acid, Cysteine, Glutamic acid, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tyrosine, Valine
Key words Amino acids, AQC
Chromatogram 1. Aspartic acid 2. Glutamic acid 3. Serine 4. Glycine 5. ? 6. Lysine (+ 1 AMQ) 7. Histidine 8. Threonine 9. Arginine 10. Alanine 11. AMQ 12. Proline 13. NH3 14. Cystine 15. Tyrosine 16. Valine 17. Methionine 18. Isoleucine 19. Leucine 20. Lysine (+2 AMQ) 21. Phenylalanine

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