The determination of carbohydrates in food and other matrices is always a topic of high concern. The exact determination of sugar content is not only essential when thinking of foods targeted to diabetics. Such sensitive analytical methods are also interesting to manufacturers of sugar based pharmaceutical products or for research projects in the field of carbohydrates. Here, we compare the HPLC analysis with electrochemical detection (ECD) versus refractive index detection (RID) for the determination of glucose, sucrose, and arabinose.
4-step PAD potential waveform for the detection of monosaccharides and other carbohydrates, the sample detection occurs during the highlighted time period tsample
ECD chromatogram of mixed sugar standard at 10 μg/mL, 1) sucrose 2) glucose 3) arabinose
RID chromatogram of mixed sugar standard at 1 mg/mL, 1) sucrose 2) glucose 3) arabinose
| Method | HPLC |
|---|---|
| Mode | Ion exclusion, Ligand exchange |
| Substances | sucrose, glucose, arabinose |
| Key words | Eurokat, Ion exclusion, Ligand exchange, ECD, electrochemical detection, pulsed amperometric detecion, RID, 4-step PAD potential, carbohydrates, sugar |
| CAS number | 57-50-1, 50-99-7, 147-81-9 |
AZURA® Pump P 6.1L (HPG), with 10 ml pump head (stainless steel), degasser and mixer (100 µl)
Article No.: APH35EA
Show product
AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 700 bar
Article No.: AAA00AA
Show product
AZURA® Detector RID 2.1L analytical refractive index detector with flow cell
Article No.: ADD31
Show product
Electrochemical detector AZURA ECD 2.1without flow cell
Article No.: A1651
Show product
SenCell 2 mm Au HyREF for Electrochemical Detector AZURA ECD 2.1, black
Article No.: A1652-3
Show product
Column thermostat AZURA CT 2.1 for constant temperatures and reproducible results
Article No.: ATC00
Show product
Article No.: 30GX210EKN
Show product
Article No.: 03GX210EKN
Show product
ClarityChrom® single instrument license for one time base
Article No.: A1670
Show product
ClarityChrom® system suitability option
Article No.: A1677
Show product
Highly sensitive analytical system with electrochemical detector, ideally suited for the determination of carbohydrates.
Article No.: A46017
Show productWe are happy to help you