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Sensitivity boost - comparison of electrochemical and refractive index detection for sugar analysis

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Application No.: VFD0183

Sensitivity boost - comparison of electrochemical and refractive index detection for sugar analysis

The determination of carbohydrates in food and other matrices is always a topic of high concern. The exact determination of sugar content is not only essential when thinking of foods targeted to diabetics. Such sensitive analytical methods are also interesting to manufacturers of sugar based pharmaceutical products or for research projects in the field of carbohydrates. Here, we compare the HPLC analysis with electrochemical detection (ECD) versus refractive index detection (RID) for the determination of glucose, sucrose, and arabinose.

Technical Data

Application details

Method HPLC
Mode Ion exclusion, Ligand exchange
Substances sucrose, glucose, arabinose
Key words Eurokat, Ion exclusion, Ligand exchange, ECD, electrochemical detection, pulsed amperometric detecion, RID, 4-step PAD potential, carbohydrates, sugar
CAS number 57-50-1, 50-99-7, 147-81-9

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