The determination of carbohydrates in food and other matrices is always a topic of high concern. The exact determination of sugar content is not only essential when thinking of foods targeted to diabetics. Such sensitive analytical methods are also interesting to manufacturers of sugar based pharmaceutical products or for research projects in the field of carbohydrates. Here, we compare the HPLC analysis with electrochemical detection (ECD) versus refractive index detection (RID) for the determination of glucose, sucrose, and arabinose.
4-step PAD potential waveform for the detection of monosaccharides and other carbohydrates, the sample detection occurs during the highlighted time period tsample
ECD chromatogram of mixed sugar standard at 10 μg/mL, 1) sucrose 2) glucose 3) arabinose
RID chromatogram of mixed sugar standard at 1 mg/mL, 1) sucrose 2) glucose 3) arabinose
| Method | HPLC |
|---|---|
| Mode | Ion exclusion, Ligand exchange |
| Substances | sucrose, glucose, arabinose |
| Key words | Eurokat, Ion exclusion, Ligand exchange, ECD, electrochemical detection, pulsed amperometric detecion, RID, 4-step PAD potential, carbohydrates, sugar |
| CAS number | 57-50-1, 50-99-7, 147-81-9 |
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