Monosaccharides belong to the most abundant group of biomolecules in nature. They play a crucial role in metabolism, structural biology, and storage of energy. Thus, the analysis of these special type of carbohydrates is of great interest for the food industry but also for a broad range of life and material sciences. The presence of hydroxyl groups enables a specific and highly sensitive analysis using pulsed amperometric detection (PAD) with the DECADE Elite electrochemical detector as part of the dedicated AZURA® High Performance Anion Exchange Chromatography (HPAEC) system.
Chromatogram of a standard mixture containing 0.1 mg/mL fucose (1), rhamnose (2), arabinose (3), galactose (4), glucose (5), xylose (6), mannose (7), galacturonic acid (8) and glucuronic acid (9). And a zoom into the peaks for the uronic acids
Concentration curves of the described sugars and uronic acids in a concentration range between 0.0125 mg/mL to 0.25 mg/mL
| Method | HPLC |
|---|---|
| Mode | IC, HPAEC |
| Substances | fucose, rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid |
| Key words | fucose, rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid, glucuronic acid, HPAEC, electrochemical detection |
| CAS number | 2438-80-4, 50-99-7, 3615-41-6, 147-81-9, 59-23-4, 58-86-6, 530-26-7, 685-73-4, 6556-12-3 |
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