Steviol glycosides are the main sweetening compounds in Stevia rebaudiana and can be used as natural sugar substitutes due to their far higher sweetening power than normal sucrose or glucose. The sweetness is estimated to be about 400 times higher. This application describes an easy transfer of an existing analytical HPLC method to a preparative HPLC using overload experiments together with the KNAUER Scale-Up converter. Furthermore, this approach of scale-up is also applicable for different analytes and thus provides a fast scale-up.
A - Transfer gradient to isocratic method; 1) rebaudioside A, 2) stevioside, 20 μL, C18, 5 μm particle; B - Overlay 100 μL (blue) and 200 μL (red) injection, C18, 10 µm particle
Overload experiments on preparative column, 200 μL (red), 500 μL (blue), 2000μL (green); 1) rebaudioside A, 2) stevioside, blue bars – matrix, 25°C, 22 ml/min
| Method | HPLC |
|---|---|
| Mode | RP |
| Substances | rebaudioside A, stevioside |
| Key words | Stevia, sweetener, steviol glycosides, online SPE, solid phase extraction, purification, preparative HPLC, sample preparation |
| CAS number | 58543-16-1, 57817-89-7 |
AZURA® Pump P 2.1L with 100 ml pump head (stainless steel)
Article No.: APE20KA
Show product
LPG module for Pump P 2.1L ternary up to 220 ml/min (stainless steel)
Article No.: AZZ00AB
Show product
AZURA® Detector UVD 2.1L with deuterium lamp without flow cell, incl. test cell
Article No.: ADA01XA
Show product
Fraction collector LABOCOL Vario-4000, for 1/16“ or 1/8“ tubing
Article No.: A591022
Show product
Preparative Column
Article No.: 25JE181E2N
Show product
Basic License for one system
Article No.: A2650
Show product
Easy-to-use method transfer software from analytical to preparative HPLC. Save all the hard work using formulas to calculate your scale up method parameters when going from analytical to preparative HPLC.
Article No.: A1696
Show productWe are happy to help you