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Is it possible to use a normal phase column in a reversed phase modus?

Yes, it is possible. NH2 normal phase columns are the most flexible ones in the normal phase family and can be used in three modes: normal phase, reversed phase and ion chromatography mode (weak anion exchanger). In reversed phase mode they are mainly used for analysis of carbohydrates. NP columns with CN stationary phases can be used for a wide range of applications in normal pase mode as well as reversed phase mode (steroids, carbohydrates polar compounds).

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

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