Nowadays sugar substitutes are used in many products, not only for diabetic purposes but to make products more attractive for customers. Furthermore, people are interested in a healthier lifestyle which includes consuming less sugar. Therefore, quality control of sugar and sugar substitutes in food and beverages is compulsory to assure the correct labelling of products and composition of ingredients. Using KNAUER Eurokat columns in combination with an electrochemical detector expands the application area of carbohydrate analysis.
Mixed standard at a concentration of 8.00 μg/mL. 1 - saccharose, 2 - sucralose, 3 - glucose, 4 - fructose, 5 - mannitol, 6 - xylitol, 7 - sorbitol
Overlay of mixed standard (red) and extracted chewing gum (blue). 1 - saccharose, 2 - sucralose, 3 - glucose, 4 - fructose, 5 - mannitol, 6 - xylitol, 7 - sorbitol
Overlay of mixed standard (red) and extracted toothpaste (blue). 1 - saccharose, 2 - sucralose, 3 - glucose, 4 - fructose, 5 - mannitol, 6 - xylitol, 7 - sorbitol
4-step PAD potential waveform for the detection of monosaccharides and other carbohydrates, the sample detection occurs during the highlighted time period tsample
Method | HPLC |
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Mode | Ion exclusion, Ligand exchange |
Substances | Saccharose, sucralose, glucose, fructose, mannitol, xylitol, sorbitol |
Key words | Carbohydrates, ECD, electrochemical detection, sugar, Eurokat, PSDVB, poymer column, PAD, pulsed amperometric detection |
CAS number | 57-50-1, 56038-13-2, 58367-01-4, 6035-50-3, 87-78-5, 87-99-0, 50-70-4 |
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