Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants and are widespread across the globe mainly due to long-term anthropogenic sources of pollution. Due to their high mutagenic and carcinogenic potential a sensitive determination is mandatory. The used system configuration makes it possible to switch between direct injection via autosampler and injection with an auxiliary pump (feed pump) without changing/replumbing the system. The use of autosampler injection or injection via feed pump enables the user to inject a broad variety of different volumes from μl to ml scale.
Exemplary structures of selected PAHs
Direct injection via autosampler of mixed standard, (1) fluoranthene, (2) benzo[b]fluoranthene, (3) benzo[k]fluoranthene, (4) benzo[a]pyrene, (5) benzo[g,h,i]perylene, (6) indeno[1,2,3-cd]pyrene
Direct injection on SPE via autosampler, (1) fluoranthene, (2) benzo[b] fluoranthene, (3) benzo[k]fluoranthene, (4) benzo[a]pyrene, (5) benzo[g,h,i] perylene, (6) indeno[1,2,3-cd]pyrene
Exemplary flow scheme for SPE load via sample/feed pump
Comparison of LOD values for direct injection (blue) and SPE injection (orange)
| Method | HPLC |
|---|---|
| Mode | RP |
| Substances | EPA 610 PAH mixture, fluoranthene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, benzo[g,h,i]perylene, indeno[1,2,3-cd]pyrene |
| Key words | HPLC, online SPE, RP, PAH, polycyclic aromatic hydrocarbons, solid phase extraction, multi-injection valve, EPA 610 |
| CAS number | 206-44-0, 205-99-2, 207-08-9, 50-32-8, 191-24-2, 193-39-5 |
| Version | Version 1/ 11/2022 |
AZURA® Pump P 6.1L (HPG), with 10 ml pump head (stainless steel), degasser and mixer (400 µl)
Article No.: APH35EA
Show product
AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 862 bar
Article No.: AAA50AA
Show product
AZURA® Detector DAD 2.1L Diode array detector DAD 2.1L without flow cell 190 - 700 nm, incl. test cell
Article No.: ADC01
Show product
50 mm path length, 6 µl, 1/16", 50 bar, High Sensitivity LightGuide Flow cell cartridge for AZURA® Detector DAD/MWD
Article No.: AMD59XA
Show product
Fluorescence detector RF-20 A 200 - 650 nm incl. accessories and flow cell
Article No.: A59200
Show product
Column thermostat AZURA CT 2.1 for constant temperatures and reproducible results
Article No.: ATC00
Show product
VU 4.1 valve drive for V 4.1 valves
Article No.: AWA01XA
Show product
Multiposition valve, 8 ports, DLC stainless steel, PEEK, 1/8“, 200 bar, 1.5 mm bore size
Article No.: AVT34AE
Show product
Left module: Pump P 4.1S, 10 ml, stainless steel - DPG22EA Middle module: Valve drive VU 4.1 - EWA04 Right module: Valve drive VU 4.1 - EWA04
Article No.: AY03231
Show product
Assistant module P 4.1S, 10 ml, stainless steel
Article No.: DPG22EA
Show product
Assistant module VU 4.1 for valves V 4.1
Article No.: EWA04
Show product
2-position valve, 8 Port, DLC stainless steel, Vespel®, 1/16", 1200 bar, 0.3 mm bore size
Article No.: AVC38AC
Show product
DLC stainless steel multi-injection valve for more sophisticated separation tasks. (1/16", 500 bar, 0.75 mm bore size)
Article No.: AVN96AE
Show product
ClarityChrom® single instrument license for one time base
Article No.: A1670
Show product
ClarityChrom® option for PDA data processing
Article No.: A1676
Show productWe are happy to help you