Liquid chromatography is a well-established technique for the separation of substances. High performance liquid chromatography (HPLC) is a suitable method for the analysis of a wide range of application areas. Here, we describe the principle of HPLC and introduce to the most important components in an HPLC system and the factors that determine the success of a measurement.
Schematic layout of a HPLC system
Analytical HPG (left) and LPG (right) system configuration with autosampler and column thermostat
Analytical and preparative column
Isocratic system configuration with a single wavelength UV-detector (left) and a refractive index detector (right)
Schematic illustration of a chromatogram
|Key words||HPLC basics, gradient, column, peak, chromatogram, HPG, LPG, HPLC principles, pump, detector, injection, HPLC system, retention time|
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