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How to use peak sampling in PurityChrom?

You can fractionate peaks using the automated peak sampling in PurityChrom. In this mode the peaks of your run are analyzed by several factors like slope and baseline and a customizable fractionation is carried out while the peak situation (e.g. peak start, peak valley, peak maximum, peak shoulder and peak end) is determined

In the time control file, you can find the tab “sampling”. Here one defines the time range in which the automatic fractionation should be active. Also, you can define which data source is used for the peak sampling and how many data points are averaged over (“filter factor”). The value for “slope sensitivity” is the most important point, because here a new peak can be identified by its steepness. The “maximum baseline level” is the value, which determines whether a peak valley or a peak end is identified. You must put in a value higher than the usual baseline of the chromatogram you want to fractionate. The settings under “maximum fractions” and “excessive fractions to” are used with specialized valves but do not apply to KNAUER hardware.

To define which actions are taken at which peak situation you click on the dot of the peak situation and then define the actions, that should be carried out by the software. To get a rough idea of the values “maximum baseline level”, “filter factor” and “slope sensitivity”, which are compatible with the peak situation of your chromatogram you can use the integration function in PurityChrom.

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

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