How to deal with talling peaks?

The problem with talling peaks can occur due to following reasons:


a) Sample reacting with active sites. Solution: First check column performance with standards. If results for test mix are good, add salts or competing base or acid modifier. b) Wrong column type. Solution: Try another column type (e.g. endcapped column for basic compounds). c) Wrong mobile phase pH. Solution: Adjust pH. For basic compounds, higher pH usually provides more symmetric peaks. d) Wrong injection solvent. Solution: Peaks can tail when sample is injected in stronger solvent than mobile phase. If possible, dissolve sample in mobile phase. e) Small void at column inlet. Solution: Repack top of column with particles of same bonded phase functionality. Continue using column in reversed flow direction.

We separate molecules and unite people.

Based in Berlin, KNAUER is a medium-sized company that has been serving the sciences since 1962. We are a developer and manufacturer of scientific instruments of superior quality for liquid chromatography (LC), liquid dosing and other laboratory tasks. The product range includes systems, engineering solutions and components for:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-column Chromatography / SMB
High-pressure dosing solutions
Lipid nanoparticle (LNP) production and research

We are an established partner in science. Today and in the future.​​​​​​

Do you need more information?

Please contact us!

Follow us