How to deal with broad peaks?

The problem with broad peaks can occur due to following reasons:


a) Mobile phase composition changed. Solution: Prepare new mobile phase. b) Mobile phase flow rate too low. Not in the optimum of van Deemter curve. Solution: Adjust flow rate. c) Leak, especially between column and detector. Peaks will be broad with lower peak height! Solution: Check system for loose fittings. Check pump for leaks, salt build-up and unusual noises. Change pump seals if necessary. d) Detector settings incorrect. Solution: Adjust settings. e) Column overloaded. Peaks will be broad with high peak height. Solution: Inject smaller volume or dilutions of sample (e.g. 1:10, 1:100). f) Detector response time or cell volume too large. Solution: Reduce response time or use smaller cell. g) Tubing between column and detector too long or ID too large. Solution: Use as short a piece of 0.007– 0.010’’ ID tubing as practical. h) Buffer concentration too low. Peaks will be broad without tailing etc. Solution: Increase concentration. i) Guard column contaminated. Solution: Replace guard column. j) Column contaminated/worn out. Solution: Replace column with new one of same type. If new column provides symmetrical peaks, flush old column and retest. k) Void at column inlet. Solution: Replace column. l) Peak represents two or more poorly resolved compounds. Solution: Change column type to improve separation. m) Column temperature too low. Solution: Increase temperature. Do not exceed recommended temperature (45 °C for Eurospher and 90 °C for Eurokat).

We separate molecules and unite people.

Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB

We are an established partner in science. Today and in the future.​​​​​​

Do you need more information?

Please contact us!

Follow us