How to deal with a regular baseline noise?





a.) Air in mobile phase, detector cell or pump.

Solution: Degas mobile phase. Flush system to remove air from detector cell or pump.

b.) Incomplete mobile phase mixing.

Solution: Mix mobile phase by hand or use less viscous solvent.

c.) Temperature effect (column at high temperature, detector unheated.)

Solution: Reduce differential or add heat exchanger.

d.) Pump pulsations.

Solution: Clean or exchange check valves of the pump head. If problem still persists, incorporate pulse damper into the system.

e.) Pressure close to maximum.
Solution: Minimize back pressure by reducing flow rate or heating the column.


We separate molecules and unite people.

Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB

We are an established partner in science. Today and in the future.​​​​​​

Do you need more information?

Please contact us!

Follow us