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How to deal with a regular baseline noise?

 

Regular

Problem

 

a.) Air in mobile phase, detector cell or pump.

Solution: Degas mobile phase. Flush system to remove air from detector cell or pump.

b.) Incomplete mobile phase mixing.

Solution: Mix mobile phase by hand or use less viscous solvent.

c.) Temperature effect (column at high temperature, detector unheated.)

Solution: Reduce differential or add heat exchanger.

d.) Pump pulsations.

Solution: Clean or exchange check valves of the pump head. If problem still persists, incorporate pulse damper into the system.

e.) Pressure close to maximum.
Solution: Minimize back pressure by reducing flow rate or heating the column.

 

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

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