How to deal with a regular baseline noise?





a.) Air in mobile phase, detector cell or pump.

Solution: Degas mobile phase. Flush system to remove air from detector cell or pump.

b.) Incomplete mobile phase mixing.

Solution: Mix mobile phase by hand or use less viscous solvent.

c.) Temperature effect (column at high temperature, detector unheated.)

Solution: Reduce differential or add heat exchanger.

d.) Pump pulsations.

Solution: Clean or exchange check valves of the pump head. If problem still persists, incorporate pulse damper into the system.

e.) Pressure close to maximum.
Solution: Minimize back pressure by reducing flow rate or heating the column.


We separate molecules and unite people.

Based in Berlin, KNAUER is a medium-sized company that has been serving the sciences since 1962. We are a developer and manufacturer of scientific instruments of superior quality for liquid chromatography (LC), liquid dosing and other laboratory tasks. The product range includes systems, engineering solutions and components for:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-column Chromatography / SMB
High-pressure dosing solutions
Lipid nanoparticle (LNP) production and research

We are an established partner in science. Today and in the future.​​​​​​

Do you need more information?

Please contact us!

Follow us