How many injections can a HPLC column withstand?

There are a number of factors that control the number of injections any modern HPLC column can withstand. Some of the factors are based upon the mode, some of these factors are based upon the type of packing in the column and again some factors are based upon the phase itself. HPLC columns could generate proper and reliable results for many years. Column care, sample, sample preparation and measuring conditions (pH, pressure, temperature) have a very strong influence on the column lifetime. It can be strongly recommeded to use a precolumn for saving column life time.

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB

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