How do I prevent column plugging in UHPLC?

To prevent column plugging, it is reasonable to filter the samples and the eluent through a filter with 2 µm pore size before usage. Furthermore the compatibility of the mobile phase is an important factor. Standard RP phases are stable within a pH range 2-8. Under certain conditions (presence of buffers) the stationary phases are even chemically stable until pH 12. When using buffered mobile phases it is necessary to flush the silica-based column with pure solvents to avoid precipitation of buffer salt. As prophylaxis KNAUER offers a universal UHPLC precolumn filter with 0.5 µm pore size.

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB

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