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How can Ion Exchange HPLC columns be cleaned and regenerated?

This is our recommended regeneration procedure of Ion Exchange packings, anion and cation exchange (WCX, SCX, WAX, SAX):

Flush the column with 20 column volumes of the same eluent,
But double the buffer concentration
Follow the regeneration protocol forRP packings
Flush the column with 20 column volumes of water
Equilibrate the column with the original conditions

We separate molecules and unite people.

Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

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