DIN ISO 11292:1995 regulates the determination and analysis of carbohydrates in instant coffee using anion exchange chromatography combined with pulsed amperometric detection (PAD). Here an adapted method is provided, using a polymer-based cation-exchange column in the Pb2+ ionic form and PAD.
Chromatogram of mixed standard with β=5.00 μg/mL. 1 - saccharose, 2 - glucose, 3 - xylose, 4 - galactose, 5 - arabinose, 6 - mannose, 7 - fructose, 8 - mannitol
Chromatogram of free hydrocarbon content. 1 - saccharose, 2 - glucose, 3 - xylose, 4 - galactose, 5 - arabinose, 6 - mannose, 7 - fructose, 8 - mannitol
Chromatogram of hydrolyzed coffee sample. 1 - glucose, 2 - xylose, 3 - galactose, 4 - arabinose, 5 - mannose, 6 - fructose, 7 - mannitol
4-step PAD potential waveform for the detection of monosaccharides and other carbohydrates. The sample detection occurs during the highlighted time period tsample
| Method | HPLC |
|---|---|
| Mode | Ion exclusion, Ligand exchange |
| Substances | Saccharose, glucose, xylose, galactose, arabinose, mannose, fructose, mannitol |
| Key words | Carbohydrates, ECD, electrochemical detection, sugar, Eurokat, PSDVB, poymer column, PAD, pulsed amperometric detection, ISO 11292:1995, coffee, instant coffee |
| CAS number | 57-50-1, 50-99-7, 25990-60-7, 59-23-4, 147-81-9, 3458-28-4, 6035-50-3, 87-78-5 |
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