Eurospher II HPLC column kit for method development in RP mode:
3 columns with the modifications C8, C18, C18A for a wide range of reversed phase selectivities.
C18: Classical Reversed Phase columns for HPLC and UHPLC
Most often used stationary phase in reversed phase mode. Silica gel based columns with C18 modification are available with or without endcapping. Classically endcapped phases are slightly more hydrophobic and unpolar than the not endcapped phases.
C18 columns are ideally used for acidic, basic and neutral analytes in reversed phase mode
C18 A or AQ: Columns for polar reversed phase applications with 100 % aqueous mobile phase
C18 stationary phase for the analysis of more polar and hydrophilic compounds in reversed phase mode (USP L1). Silica gel based columns with C18 modification with hydrophilic endcapping. Because of their modification, C18A or C18 aq phases can be operated under 100 % aqueous conditions without destroying the stationary phase, what is not recommended for classical C18 phases caused by their hydrophobicity.
Classical C8 columns for HPLC and UHPLC
C8 is a silica gel based stationary phase in reversed phase mode with alternative selectivity compared to C18 phases. C8 modifications are available with a slightly hydrophobic endcapping or without endcapping.
Compared to C18 phases, these columns show less retention due to the lower hydrophobicity.
These phases are ideal for the analysis of:
- Water soluble vitamins
Dimension 250 x 4,6 mm ID, 5 µm particles
Recommended for classical HPLC systems with 400 bar maximum backpressure