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Eurospher II RP analytical Column Kit 5 µm: C8, C18, C18A in 250 x 4.6 mm column with integrated precolumn

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Article No.: A66250RP
Articlename: Eurospher II RP analytical Column Kit 5 µm: C8, C18, C18A in 250 x 4.6 mm column with integrated precolumn

Eurospher II RP analytical Column Kit 5 µm: C8, C18, C18A in 250 x 4.6 mm column with integrated precolumn


for method development

Eurospher II HPLC column kit for method development in RP mode:

3 columns with the modifications C8, C18, C18A for a wide range of reversed phase selectivities.

C18: Classical Reversed Phase columns for HPLC and UHPLC 
Most often used stationary phase in reversed phase mode. Silica gel based columns with C18 modification are available with or without endcapping. Classically endcapped phases are slightly more hydrophobic and unpolar than the not endcapped phases.
C18 columns are ideally used for acidic, basic and neutral analytes in reversed phase mode

C18 A or AQ: Columns for polar reversed phase applications with 100 % aqueous mobile phase
C18 stationary phase for the analysis of more polar and hydrophilic compounds in reversed phase mode (USP L1). Silica gel based columns with C18 modification with hydrophilic endcapping. Because of their modification, C18A or C18 aq phases can be operated under 100 % aqueous conditions without destroying the stationary phase, what is not recommended for classical C18 phases caused by their hydrophobicity.

Classical C8 columns for HPLC and UHPLC 
C8 is a silica gel based stationary phase in reversed phase mode with alternative selectivity compared to C18 phases. C8 modifications are available with a slightly hydrophobic endcapping or without endcapping. 
Compared to C18 phases, these columns show less retention due to the lower hydrophobicity. 
These phases are ideal for the analysis of:

  • Water soluble vitamins
  • Steroids
  • Catecholamines
  • Sedatives

Dimension 250 x 4,6 mm ID, 5 µm particles

Recommended for classical HPLC systems with 400 bar maximum backpressure

Technical Data

Separation column

Brand Eurospher II
Particle size 5 µm
Pore size 100 Å
USP code L1
Length [mm] 250 mm
Inner diameter [mm] 4.6 mm with integrated precolumn
Connection 1/16"
Pore volume 0.8 ml/g
Metal content < 10 ppm
Density 430 g/l
Temperature range up to 40°C (up to 50 °C short term)
Specific surface area 320 ± 20 m²/g

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