Eurospher II HPLC column kit for method development in RP mode:
3 columns with the modifications C18, C18H, C18A for a wide range of reversed phase selectivities.
C18: Classical Reversed Phase columns for HPLC and UHPLC
Most often used stationary phase in reversed phase mode. Silica gel based columns with C18 modification are available with or without endcapping. Classically endcapped phases are slightly more hydrophobic and unpolar than the not endcapped phases.
C18 columns are ideally used for acidic, basic and neutral analytes in reversed phase mode
C18 A or AQ: Columns for polar reversed phase applications with 100 % aqueous mobile phase
C18 stationary phase for the analysis of more polar and hydrophilic compounds in reversed phase mode (USP L1). Silica gel based columns with C18 modification with hydrophilic endcapping. Because of their modification, C18A or C18 aq phases can be operated under 100 % aqueous conditions without destroying the stationary phase, what is not recommended for classical C18 phases caused by their hydrophobicity.
C18 H: Columns for more unpolar analytes in Reversed Phase
C18 stationary phase with a special, high efficiency or polymeric unpolar endcapping style. Because of the high carbon content and the protection of the Silica gel these materials are also suited for the use of mobile phase in an extended pH range from typically pH 1-12.
While a high endcapped C18 phase is characterized mostly by the high carbon content, a polymeric endcapped phase also provides an increased steric selectivity.
Dimension 150 x 4 mm ID, 5 µm particles
Recommended for classical HPLC systems with 400 bar maximum backpressure