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Do I need to use a gradient with UHPLC columns?

It is not mandatory required to run a gradient analysis. Dependent on what you want to separate, isocratic applications are of course possible. The use of gradient elution is more common because of shorter analysis times.

We separate molecules and unite people.

Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

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