Do I need to use a gradient with UHPLC columns?

It is not mandatory required to run a gradient analysis. Dependent on what you want to separate, isocratic applications are of course possible. The use of gradient elution is more common because of shorter analysis times.

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB

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