Determination of Neomycin sulphate

Application No.: VPH0004J

Determination of Neomycin sulphate

Neomycin is an aminoglycoside antibiotic found in many topical medications such as creams, ointments, and eyedrops. Standard grade neomycin is composed of a number of related compounds including neomycin A (neamine), neomycin B (framycetin), neomycin C, and a few minor compounds found in much lower quantities. Neomycin B is the most active component in neomycin followed by neomycin C and neomycin A. Neomycin A is an inactive degradation product of the C and B isomers. The quantities of these components in neomycin vary from lot-to-lot depending on the manufacturer and manufacturing process.

Technical Data


Method HPLC

Separation conditions

Mode RP
Substances Neomycin sulphate B, Neomycin sulphate C
Key words Neomycin sulphate, aminoglycoside antibiotics, ELSD
CAS number 1404-04-2

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