A fast gradient method for the separation and quantification of six substances used as monomers for the production and development of biocompatible polymers is presented in this application note. The high speed and reliability of this method applying the KNAUER UHPLC System make it well-suited as well for routine analyses as for research tasks. The very short analysis time of less than 7 minutes is achieved by employing the Eurospher (former Bluespher) phenyl stationary phase with a 2 µm particle size filled in a 2 mm ID column. A binary high pressure gradient instrumentation is used at a flow rate of 0.3 ml/min in combination with the UV-detector.
1 2-Hydroxyethyl methacrylate; 2 N-Vinyl-2-pyrrolidon; 3 Methyl methacrylate; 4 Ethylenglycol dimethacrylate; 5 Styrene; 6 Methacryloxymethyl-trimethylsilan
|Substances||2-Hydroxyethylmethacrylate, N-Vinyl-2-pyrrolidon, Methyl methacrylate, Ethylenglycon dimethacrylate, Styrene, Methacryloxymethyl trimethylsilane|
|Key words||Biopolymer, Biomaterial, Implants, Monomer, Hydrogel|
|CAS number||868-77-9, 88-12-0, 80-62-6, 97-90-5, 100-42-5, 18269-97-1|
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