EN DE

Determination of 15 PAH with Fluorescence detection (copy 1)

Contact us
Application No.: VEV0054J

Determination of 15 PAH with Fluorescence detection (copy 1)

BlueOrchid PAH 150 x 2 mm ID

Order No. 15BF420BOG

Vertex Plus column, 30x2 mm, 50x2 mmFor a link to the column see below: Related products

Technical Data

Application

Method UHPLC

Separation conditions

Eluent A: MeOH/H2O 75:25B: Acetonirtrile
Gradient 0.0 – 2.5 min 10 % B 2.5 – 6.0 min 10 – 100 % B 6.0 – 10.0 min 100 % B
Flow rate 0.6 ml/min
Injection volume 10 μl standard, 10 μg/L
Temperature 25 °C
Detection Fluorescence RF-20A XS, wavelength program: 0.0 – 3.0 min 270 Ex, 330 Em 3.0 – 4.6 min 250 Ex, 370 Em 4.6 – 5.8 min 330 Ex, 430 Em 5.8 – 7.0 min 270 Ex, 390 Em 7.0 – 8.9 min 290 Ex, 430 Em 8.9 – 12.0 min 370 Ex, 460 Em 12.0 – 15.0 min 270 Ex, 330 Em
Chromatogram 1. Naphthalene 2. Acenapthene 3. Fluorene 4. Phenanthrene 5. Anthracene 6. Fluoranthene 7. Pyrene 8. Benzo(a)anthracene 9. Chrysene 10. Benzo(b)fluoranthene 11. Benzo(k)fluoranthene12. Benzo(a)pyrene13. Dibenzo(a,h)anthracene14. Benzo(g,h,i)perylene15. Indeno(1,2,3-cd)pyren

Documents and more information

Do you need more details or application support?

We are happy to help you

Follow us