Reversed Phase (RP) is with more than 90 % market share by far the most commonly used HPLC mode. The best known and most used surface modification is C18. Although the USP column code (L1) is the same for all C18 phases, there are a lot of differences which must be considered when choosing the right column. C18 always is a good choice for an initial try but one must bear in mind that not all C18 phases have the same separation characteristics. To differentiate between such phases Tanaka plots are extremely useful.
Tanaka Plots of Eurospher II 100-5 C18 (left) and Eurospher 100-5 C18 A (right)
Tanaka plots of Eurospher II 100-5 C18 H (left) and Eurospher II 100-5 C18 P (right)
Tanaka plots of Eurospher I 100-5 C18 (left) and Eurosil Bioselect 300-5 C18 (right)
| Method | HPLC |
|---|
| Mode | RP |
|---|---|
| Substances | uracil, butyl benzene, o-terphenylen, pentyl benzene, triphenylen |
| Key words | Tanaka, reversed phase, column characterization, C18, Tanaka plot, hydrophobic retention, shape selectivity |
| CAS number | 66-22-8, 104-51-8, 84-15-1, 538-68-1, 217-59-4 |
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