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Can the HPLC column be operated in reversed flow direction?

Every column has a preferred flow direction. The flow direction of KNAUER columns is indicated with an arrow on the column label. This is also the direction the column was packed. It is possible to run the column in reversed flow direction but the filters on the columns inlet and outlet need to have the same pore size. It is not assured that the column will provide the same quality when operated in reversed direction.

We separate molecules and unite people.

Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

We are an established partner in science. Today and in the future.​​​​​​

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