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Are UHPLC columns more efficient?

UHPLC columns are contingently more efficient. You will get the same separation when scaling down linear from HPLC to UHPLC: 250 x 4.6 mm ID (5 µm particle) → 150 x 4 mm ID (3 µm particle) → 100 x 2 mm ID (2 µm particle) For more effiency relating to theoretical plate numbers the separation distance has to be longer as used for linear scale down.
Furthermore if the extracolumn contributions to band broadening of a particular HPLC hardware system are high then the full performance of this column will not be realized.

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

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