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Advantages of preparative online SPE compared to batch LC for stevia purification

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Advantages of preparative online SPE compared to batch LC for stevia purification
Application No.: VFD0171

Steviol glycosides are the main sweetening compounds in Stevia rebaudiana. Due to their up to 400 times higher sweetening power compared to sucrose or glucose they are often used as natural sugar substitutes. To enable a commercial usage, the plant extracts need to be purified. In this work preparative online SPE (solid phase extraction) was investigated for improvement of overall purity due to reduction of matrix contamination.

Technical Data

Application details

Method Preparative HPLC
Mode RP; online SPE
Substances rebaudioside A, stevioside
Key words Stevia, sweetener, steviol glycosides, online SPE, solid phase extraction, purification, preparative HPLC, sample preparation
CAS number 58543-16-1, 57817-89-7

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