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Evaluating preparative online SPE for the purification of stevia leave extracts

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Application No.: VFD0171

Evaluating preparative online SPE for the purification of stevia leave extracts

In order to enable commercial use in the quality assurance of the crude products and for the production of high-purity reference compounds, the plant extract must be purified, since matrix components stress the separation column and reduce the loadability.

In this application, we are investigating the matrix removal efficiency of preparative online SPE and the achievable yield of pure rebaudioside A and stevioside.

Steviol glycosides are the main sweetening compounds in Stevia rebaudiana. Due to their up to 400 times higher sweetening power compared to sucrose or glucose they are often used as natural sugar substitutes.

 

 

Technical Data

Application details

Method Preparative HPLC
Mode RP; online SPE
Substances rebaudioside A, stevioside
Key words Stevia, sweetener, steviol glycosides, online SPE, solid phase extraction, purification, preparative HPLC, sample preparation
CAS number 58543-16-1, 57817-89-7

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