Special HPLC system for analysis of carbonyl emissions in airIn the monitoring of industrial air, the determination of carbonyl (aldehyde and ketone) emissions is crucial to prevent respiratory, pulmonological, autoimmune diseases, and cancer.
According to the analytical method described in DIN ISO 16000-3, the carbonyls must be converted to their corresponding hydrazones with 2,4-dinitrophenylhydrazine (DNPH) in order to be detected via UV detector and analyzed by reversed phase HPLC. For method optimization DryLab® has been used.
KNAUER designed a special HPLC system for analysis of carbonyl emissions in air. This gradient HPLC system perfectly meets even sophisticated analysis demands with outstanding robustness and quality “Made in Germany”.
The DNPH method is suitable for the determination of aliphatic and aromatic aldehydes as well as ketones. The air samples are drawn via cartridges featuring a surface, modified with 2,4-dinitrophenyl hydrazine (DNPH). As samples pass through the cartridge, the carbonyls in the air derivatize to their corresponding hydrazones, which show strong UV activity. The hydrazones are eluted from the cartridge with acetonitrile and can then be separated by HPLC. A well-suited detection wavelength is at 360 nm.
The more carbonyl DNPH derivatives are to be determined in one run, the higher the requirements for resolution and robustness of the separation method.
• Quaternary gradient HPLC system
• Sensitive 8-channel DAD detector
• Pump unit (max. 700 bar)
• Wide selection of supported software packages (optional)
• Leakage management system
• Mobile Control for monitoring and control of the integrated modules
Max. flow rate: 10 ml/min
Flow rate range: 0.001 - 10 ml/min
Flow rate increment: 0.001 ml/min
Maximum delivery pressure: 10150 psi / 700 bar / 70 MPa
Pressure range: 10 ml pump head stainless steelrecommended (continuous)
Pump head materials: Stainless Steel
Gradient: Low pressure gradient (quaternary)
AZURA Pump P 6.1L
Detector type: Diode array detector
Operation Area: Analytical HPLC, Preparative HPLC, Analytical UHPLC, FPLC
Detection channels: 8 (Digital)/4 (Analog)
Wavelength range: 190 - 700 nm
Number of diodes: 256
Linearity: > 2.0 AU at 274 nm
Detectors: AZURA Detector DAD 2.1L
Fast and very versatile analytical HPLC autosampler
Maximum back pressure: 700 bar
0.1-10 µl injection volume
Sample loop: 100 µlView autosampler
Autosampler AZURA AS 6.1L
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