Test 13 Carbonyl DNPH derivatives at the same time

According to the analytical method described in DIN ISO 16000-3, the carbonyls must be converted to their corresponding hydrazones with 2,4-dinitrophenylhydrazine (DNPH) in order to be detected via UV detector and analyzed by reversed phase HPLC. For method optimization DryLab® has been used. 
KNAUER designed a special HPLC system for analysis of carbonyl emissions in air. This gradient HPLC system perfectly meets even sophisticated analysis demands with outstanding robustness and quality “Made in Germany”.

The DNPH method

The DNPH method is suitable for the determination of aliphatic and aromatic aldehydes as well as ketones. The air samples are drawn via cartridges featuring a surface, modified with 2,4-dinitrophenyl hydrazine (DNPH). As samples pass through the cartridge, the carbonyls in the air derivatize to their corresponding hydrazones, which show strong UV activity. The hydrazones are eluted from the cartridge with acetonitrile and can then be separated by HPLC. A well-suited detection wavelength is at 360 nm.

The more carbonyl DNPH derivatives are to be determined in one run, the higher the requirements for resolution and robustness of the separation method.

HPLC system requirements

  • System data
  • Pump
  • Detector
  • Autosampler
  • HPLC solution by KNAUER

    • Quaternary gradient HPLC system
    • Sensitive 8-channel DAD detector
    • Pump unit (max. 700 bar)
    • Wide selection of supported software packages (optional)
    • Leakage management system
    • Mobile Control for monitoring and control of the integrated modules

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    analytical HPLC special system offer

  • AZURA P 6.1L High Pressure Pump with 10 ml pump head, stainless steel

    Max. flow rate: 10 ml/min
    Flow rate range: 0.001 - 10 ml/min
    Flow rate increment: 0.001 ml/min
    Maximum delivery pressure: 10150 psi / 700 bar / 70 MPa
    Pressure range: 10 ml pump head stainless steelrecommended (continuous) 
    Pump head materials: Stainless Steel
    Gradient: Low pressure gradient (quaternary)

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    AZURA Pump P 6.1L

  • AZURA Detector DAD 2.1L

    Detector type: Diode array detector
    Operation Area: Analytical HPLC, Preparative HPLC, Analytical UHPLC, FPLC
    Detection channels: 8 (Digital)/4 (Analog)
    Wavelength range: 190 - 700 nm
    Number of diodes: 256
    Linearity: > 2.0 AU at 274 nm

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    Detectors: AZURA Detector DAD 2.1L

  • Autosampler

    Fast and very versatile analytical HPLC autosampler

    Maximum back pressure: 700 bar 

    0.1-10 µl injection volume

    Sample loop:    100 µl

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    Autosampler AZURA AS 6.1L

    Autosampler AZURA AS 6.1L

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Special system offer

High precision components ensure sensitive analysis and reilable results. This HPLC system solution for the determination of airborne carbonyls is highly specialized, but of course can be used for many more applications. Let your application define your system solution!

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