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Special FPLC system for ion exchange chromatography (IEC)

Complete solutions for FPLC on a small footprint.
AZURA FPLC systems combine flexibility and reliability. The biocompatible and metal-free FPLC system is the perfect choice for your protein purification task.
IEC is a popular technique in protein purification. AZURA Bio Lab system supports all gradient methods including ion exchange chromatography.

IEC solution by KNAUER

Ion exchange chromatography separates proteins based on the overall charge of the molecules. The initial binding takes place under low ionic strength conditions. Elution is achieved by a salt gradient. By increasing the salt concentration proteins with a weak negative charge elute first, while at higher salt concentrations proteins with a strong negative charge elute later. A precise gradient formation is here one important parameter for a successful separation of proteins. AZURA Bio Lab system supports all gradient methods including ion exchange chromatography.

Ion exchange chromatography (IEC) method

Ion exchange chromatography separates molecules based on the overall charge of the protein. The proteins of interest have a charge opposite to that of the resin. In the case of cation exchange chromatography, proteins have an overall negative charge while binding to a cationic column (see image).
The initial binding takes place under low ionic strength conditions. Elution is achieved by a salt gradient. By increasing the salt concentration proteins with a weak negative charge elute first, while at higher salt concentrations proteins with a strong negative charge elute later. Ion exchange chromatography is frequently used for protein purification. A precise gradient formation is here one important parameter for a successful separation of proteins. AZURA Bio Lab System supports all gradient methods including ion exchange chromatography.

Separation of three model proteins by a salt gradient 

Chromatogram of the separation of three model proteins with cation exchange chromatography
Blue line: UV 280 nm signal
Red line: conductivity signal
1) peak containing α-Chymotrypsinogen A,
2) peak containing Cytochrome C
3) peak containing Lysozyme

FPLC system requirements

  • System data
  • Pump
  • Detector
  • Conductivity Monitor
  • FPLC system for Ion Exchange Chromatography (IEC) 

    • High pressure gradient pump (50ml/min), metal-free
    • Fraction collector
    • Injection valve incl. 500 μl sample loop
    • UV detector with variable wavelength and conductivity monitor
    • Compatible with all common columns and FPLC methods
    • Intuitive PurityChrom® software

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  • AZURA P 6.1L High Pressure Pump with 50 ml pump head, Ceramic, without Degasser

    Max. flow rate: 50 ml/min
    Flow rate range: 0.001 - 50 ml/min
    Flow rate increment: 0.001 ml/min
    Maximum delivery pressure: 2900 psi / 200 bar / 20 MPa
    Pressure range: 50 ml pump head ceramic recommended (continuous) 
    Pump head materials: Ceramic
    Gradient: High pressure gradient (quaternary)

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    AZURA Pump P 6.1L

  • AZURA Detector UVD 2.1S

    Detector type: Small variable single wavelength
    Operation area: Analytical HPLC, Preparative HPLC, Analytical UHPLC, FPLC
    Detection channels: 1
    Light source:  Deuterium (D²) lamp with integrated GLP chip
    Wavelength range: 190–500 nm
     
    Spectral bandwidth:    13 nm at Hα line (FWHM)

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    AZURA UVD 2.1S detector

  • Conductivity Monitor

    Measurement accuracy: 

    • Temperature sensor accuracy: ±1.0 °C;
    • Conductivity accuracy: <5 % full scale end value
    • pH accuracy: ±0.5 pH in temperature range of 4–25 °C

    Measurement precision: Conductvity precision measured in range 0.1–300 mS/cm: <2 % of end value or ≤5 mS/cm of higher values pH precision ±0.2 pH in temperature range 4–25°C
    Measurement range:  0.1–999mS/cm
    pH measurement:  2–12
    Maximum data rate: 5 Hz
    Flow cell: 10 ml or 100 ml

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    AZURA CM 2.1S

Special offer for a limited time only!

A very special FPLC system for a very special price.
Suitable for all gradient methods including ion exchange chromatography - Let your application define your system solution!

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