The C4 modification is the most often used wide pore phase for the analysis of proteins, because most of them are well separated on these stationary phases
Ultra pure, spherical wide pore HPLC phase based on wide pore silica gel
Unpolar, monomeric C4 modification, endcapped
2 % carbon content (conventional endcapping)
Properties:
Separation mechanism: hydrophobic and hydrophilic interaction
Key features:
Eurosil Bioselect 300 C4 has been specifically developed for the determination and purification of peptides, proteins, and oligonucleotides up to MW approx. 100,000 Da. The optimized manufacturing process guarantees excellent batch–to-batch reproducibility. The Eurosil Bioselect sorbent is the first choice for Biochromatography with silica material.
Technical data:
C4 phase based on ultra-pure silica gel (purity > 99 %), metal content < 10 ppm, pH-stability 2 - 8, pore size 300 A, particle size 3 μm, 5 μm, 10 μm (15 μm, 20 – 45 μm on request)
Recommended application areas:
hydrophilic biomolecules and especially peptides larger than 10,000- 20,000 Da
ProntoSIL C4 is an ultra pure, spherical wide pore HPLC phase based on wide pore silica gel. The material is unpolar, with a monomeric C4 modification and no endcapping.
The ProntoSIL C4 is a classical C4 type stationary phase. Due to the bonding technology it shows an enhanced stability even at pH 1. The C4 packings show excellent properties for the separation of large biomolecules like proteins and peptides not only in the RP-mode but also in the HIC-mode (Hydrophobic Interaction Chromatography). The C4 bonding type is available in several pore sizes and in particle sizes of 3 μm, 5 μm and 10 μm.
