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The right sample solution for a given combination of KNAUER modules

A KNAUER System Check (SC) is recommended for testing newly installed AZURA® systems as well as for regularly monitoring the system performance.

A part of the SC is the so-called "Injection and detection test". For this test procedure a certain sample concentration and volume is required for a given detector or monitor.

However, as the detector is installed in a system, the sample plug reaching the detector is affected by upstream located modules, tubings, and connections. This makes it tricky to predict a concrete sample property for arbitrary system configurations.

With the following collection KNAUER provides an overview of sample concentrations and volumes for various constellations that found to be working in daily praxis.

If you do not find your configuration just estimate from configurations given in the table or contact our support team for help.
If you tested a sample successfully under conditions not present in the table or were not able to receive a positive test result with a listed one, please share this information with us (support@knauer.net) so we can nurture this collection!
 

System pump
pump head
Type of detection Flow cell
characteristic
Analyte Sample size
[µl]
Analyte
concentration
Tested by
10 ml UV 0.5 mm Acetone 10 tbd KNAUER
UV 0.5 mm Acetone 100 tbd ...
UV 3 mm Acetone 10 tbd  
UV 3 mm Acetone 100 tbd  
Conductivity 30 µl NaCl 10 tbd  
Conductivity 300 µl NaCl 100 tbd  
FLD all FITC 10 tbd  
FLD all FITC 100 tbd  
RID analytic Acetone 10 tbd  
RID analytic Acetone 100 tbd  
...          
50 ml UV 0.5 mm Acetone 10 tbd  
UV 0.5 mm Acetone 100 tbd  
UV 3 mm Acetone 10 tbd  
UV 3 mm Acetone 100 tbd  
Conductivity 20 µl NaCl 10 tbd  
Conductivity 300 µl NaCl 100 tbd  
FLD all FITC 10 tbd  
FLD all FITC 100 tbd  
RID analytic Acetone 10 tbd  
RID analytic Acetone 100 tbd  
...          
100 ml ...          
...          
...          
250 ml ...          
500 ml ...          
1 000 ml ...          

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