Osmometry is an advanced analytical method for determining the osmotic concentration of solutions. The osmotic – or solute – concentration of a colloidal system is expressed in osmoles (Osm) per unit of volume (Osm/L) or weight (Osm/kg). In clinical and formulation contexts, osmolarity/osmolality refers to solutes as small as ions which require exceptional sensitivity down to the milliosmole (mOsm) range. At such scales, the minute physiological conditions of solutions matter greatly.
In this article, KNAUER explores some of the basic principles of osmometry in more detail.
Osmometry: Introducing tonicity
By measuring the total solute concentration of samples, osmometers can define the extent to which a solution can move water through a semipermeable membrane via osmosis. This is called tonicity. There are three underlying types of tonicity in osmometry; each expressed as relative to a solution separated by a semipermeable membrane:
KNAUER is one of the world’s leading specialists in reliable and user-friendly freezing point osmometry for a range of markets. Our K-7400S is a flexible solution for measuring the osmotic concentration of a wide range of sample types. If you would like more information, contact us today.
The ease-of-usability associated with freezing point osmometry has opened a range of avenues of application beyond healthcare and pharmaceutical markets. At KNAUER, we provide reliable osmometers for food analysis, pharmaceuticals, and various research-based applications.