How to improve preparative HPLC by mass-directed fractionation
In preparative HPLC, it is common to monitor and control the purification via refractive index or UV signal. Although both detection principles can detect peaks, they are not specific enough to identify, for example, co-elution.
Exact analysis is usually carried out after separation by mass spectrometry.
Applying a mass spectrometer (MS) already during the preparative purification is therefore a promising option. It allows both a more accurate fractionation and the analysis directly at the separation.
The compact mass spectrometer 4000 MiD is perfectly suited for this purpose. It represents a perfect complement to preparative standard HPLC systems with its small footprint, integrated vacuum pump and easy handling.